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- Title
Genetic Diversity Assessment in Trachyspermum ammi L. Sprague Using CDDP and CBDP Markers.
- Authors
Tomar, Priya; Malik, C. P.
- Abstract
Trachyspermum ammi.L. Sprague commonly known as 'Ajwain' belonging to family Apiaceae is distributed throughout India and is mostly cultivated in Gujarat and Rajasthan which constitutes various phytochemical constituents and proven to possess various pharmacological activities. Genetic diversity study was carried out in twelve accessions of Trachyspermum ammi by using Conserved DNA-Derived Polymorphism (CDDP) and CAAT Box-Derived Polymorphism (CBDP) markers. Total genomic DNA was extracted from young leaves following the standard CTAB method. A total of 21 CDDP primers and 25 CBDP primers were used for the analysis. In CDDP, method uses single 15-19 primers for PCR at annealing temperature of 50°C while CBDP primers are 18 nucleotides long but with same annealing temperature 50°C. The present investigation revealed that these novel molecular markers could be successfully utilized to determine genetic diversity and genetic relationship of various accessions of Trachyspermum ammi.
- Subjects
BISHOP'S-weed; GENETIC polymorphisms in plants; GENETIC markers in plants; DNA primers; DNA derivatives
- Publication
Journal of Plant Science Research, 2016, Vol 32, Issue 1, p27
- ISSN
0970-2539
- Publication type
Article