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- Title
Mitogenic Properties of Fab and F(ab')<sub>2</sub> Fragments of Rabbit Anti-Human β2-Microglobulin for Human Lymphocytes.
- Authors
Ringden, O.; Johnsson, B. G.
- Abstract
Bivalent F(ab')a fragments and monovalent Fab fragments of rabbit anti-human β2-microglobulin (anti-β2m) stimulated DNA synthesis in human lymphocytes. Mitogenicity of anti-β2m antibodies can therefore be ascribed to the antigenbinding site and not to the Fc portion of the molecule. The mitogenic response to F(ab')2 and sometimes Fab, fragments of anti-flam IgG was comparable to that obtained with the original IgG antibodies when tested at the same protein concentration. Since Fab monomers of anti-β2m can cause lymphocyte activation, 'cross-linking' of hypothetical β2-microglobulin-containing lymphocyte receptors does not seem necessary for activation. F(ab')2, as well as Fab, fragments of anti-fiam blocked the cytotoxic effect of anti-β2m IgG, showing that the fragments did indeed react with β2-microglobulin on the cell surface. F(ab')2 dimers, but not Fab monomers, of anti-β2m were capable of inhibiting the cytotoxic effect of an anti-HLA-A2 antiserum, The mitogenic activity of both anti-flam IgG and Fab monomers of such antibodies disappeared after absorption with highly purified β2-microglobulin. The mitogenic effect of anti-Sam IgG was inhibited to a minor extent by exposure of cells to high concentrations of pooled multispecific anti-HLA antibodies. This effect was probably nonspecific.
- Subjects
LYMPHOCYTES; LABORATORY rabbits; IMMUNOGLOBULIN G; IMMUNOGLOBULINS; MONOMERS; DNA
- Publication
Scandinavian Journal of Immunology, 1977, Vol 6, Issue 4, p281
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1977.tb00395.x