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- Title
Genetic Characterization of Lumpy Skin Disease Viruses Circulating in Lesotho Cattle.
- Authors
Makalo, Mabusetsa Joseph Raporoto; Settypalli, Tirumala Bharani Kumar; Meki, Irene Kasindi; Bakhoum, Mame Thierno; Ahmed, Hatem Ouled; Phalatsi, Moeketsi Solomon; Ramatla, Tsepo; Onyiche, ThankGod Emmanuel; Nionzima-Bohloa, Lineo; Metlin, Artem; Dhingra, Madhur; Cattoli, Giovanni; Lamien, Charles Euloge; Thekisoe, Oriel Matlhahane Molifi
- Abstract
Lumpy skin disease is one of the fast-spreading viral diseases of cattle and buffalo that can potentially cause severe economic impact. Lesotho experienced LSD for the first time in 1947 and episodes of outbreaks occurred throughout the decades. In this study, eighteen specimens were collected from LSD-clinically diseased cattle between 2020 and 2022 from Mafeteng, Leribe, Maseru, Berea, and Mohales' Hoek districts of Lesotho. A total of 11 DNA samples were analyzed by PCR and sequencing of the extracellular enveloped virus (EEV) glycoprotein, G-protein-coupled chemokine receptor (GPCR), 30 kDa RNA polymerase subunit (RPO30), and B22R genes. All nucleotide sequences of the above-mentioned genes confirmed that the PCR amplicons of clinical samples are truly LSDV, as they were identical to respective LSDV isolates on the NCBI GenBank. Two of the elevem samples were further characterized by whole-genome sequencing. The analysis, based on both CaPV marker genes and complete genome sequences, revealed that the LSDV isolates from Lesotho cluster with the NW-like LSDVs, which includes the commonly circulating LSDV field isolates from Africa, the Middle East, the Balkans, Turkey, and Eastern Europe.
- Subjects
LESOTHO; LUMPY skin disease; VIRUS diseases; WHOLE genome sequencing; CATTLE genetics; CATTLE; CATTLE diseases; CHEMOKINE receptors
- Publication
Viruses (1999-4915), 2024, Vol 16, Issue 5, p762
- ISSN
1999-4915
- Publication type
Article
- DOI
10.3390/v16050762