We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Microbial community analysis of rectal methanogens and sulfate reducing bacteria in two non-human primate species.
- Authors
Nakamura, Noriko; Leigh, Steven R.; Mackie, Roderick I.; Gaskins, H. Rex
- Abstract
Background Methanogenesis by methanogenic Archaea and sulfate reduction by sulfate reducing bacteria (SRB) are the major hydrogenotrophic pathways in the human colon. Methanogenic status of mammals is suggested to be under evolutionary rather than dietary control. However, information is lacking regarding the dynamics of hydrogenotrophic microbial communities among different primate species. Methods Rectal swabs were collected from 10 sooty mangabeys ( Cercocebus atys) and 10 baboons ( Papio hamadryas). The diversity and abundance of methanogens and SRB were examined using PCR-denaturing gradient gel electrophoresis (DGGE) and real-time quantitative PCR (qPCR). Results The DGGE results revealed that intestinal Archaea and SRB communities differ between mangabeys and baboons. Phylogenetic analyses of Archaea DGGE bands revealed two distinct clusters with one representing a putative novel order of methanogenic Archaea. The qPCR detected a similar abundance of methanogens and SRB. Conclusions Intestinal Archaea and SRB coexist in these primates, and the community patterns are host species-specific.
- Subjects
ARCHAEBACTERIA; BACTERIA; COLON (Anatomy); DENATURING gradient gel electrophoresis; BABOONS
- Publication
Journal of Medical Primatology, 2009, Vol 38, Issue 5, p360
- ISSN
0047-2565
- Publication type
Article
- DOI
10.1111/j.1600-0684.2009.00361.x