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- Title
Reduction of oxidative stress improves insulin signaling in cardiac tissue of obese mice.
- Authors
Scarpatto Rodrigues, Matheus; da Silva Pieri, Bruno Luiz; de Bem Silveira, Gustavo; Pereira Zaccaron, Rubya; Milanez Venturini, Ligia; Comin, Vitor Hugo; Damian Luiz, Karine; Lock Silveira, Paulo Cesar
- Abstract
Objective: To evaluate the effects of oxidative stress on insulin signaling in cardiac tissue of obese mice. Methods: Thirty Swiss mice were equally divided (n=10) into three groups: Control Group, Obese Group, and Obese Group Treated with N-acetylcysteine. After obesity and insulin resistance were established, the obese mice were treated with N-acetylcysteine at a dose of 50mg/kg daily for 15 days via oral gavage. Results: Higher blood glucose levels and nitrite and carbonyl contents, and lower protein levels of glutathione peroxidase and phosphorylated protein kinase B were observed in the obese group when compared with their respective control. On the other hand, treatment with N-acetylcysteine was effective in reducing blood glucose levels and nitrite and carbonyl contents, and significantly increased protein levels of glutathione peroxidase and phosphorylated protein kinase B compared to the Obese Group. Conclusion: Obesity and/or a high-lipid diet may result in oxidative stress and insulin resistance in the heart tissue of obese mice, and the use of N-acetylcysteine as a methodological and therapeutic strategy suggested there is a relation between them.
- Subjects
OXIDATIVE stress; INSULIN resistance; CELLULAR signal transduction; MYOCARDIUM; ACETYLCYSTEINE; LABORATORY mice
- Publication
Einstein (16794508), 2020, Vol 18, p1
- ISSN
1679-4508
- Publication type
Article
- DOI
10.31744/einstein_journal/2020AO5022