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- Title
Somaclonal variation in Dezful cultivar of olive (Olea europaea subsp. europaea).
- Authors
Farahani, Farah; Yari, Reza; Masoud, Sheidai
- Abstract
Tissue culture of two cultivars Dezful Baghe-Malek and Dezful Safiabad (olea europaea L.) were performed using internode cuttings. The experiment was carried out up to the seventh subculture to study the somaclonal variations. Significant difference was observed in morphological characters among the regenerated plants of different subcultures in both cultivars. Grouping of the genotypes based on morphological data separated regenerated plants of the latter subcultures from the earlier ones due to their morphological differences. RAPD analysis produced polymorphic and specific bands in the genotypes studied. Grouping of the genotypes based on RAPD data revealed that in both cultivars the parental genotype and regenerated plants of the first subculture are placed close to each other while the regenerated plants of the sixth and seventh subcultures stand far from the other genotypes due to their genetic difference. No significant difference occurred in C-value in mother plants and the regenerated plants, indicating that genetic differences are mainly of nucleotide polymorphism/mutations and not due to quantitative change in DNA amount during tissue culture process.
- Subjects
TISSUE culture; CULTIVARS; OLIVE; PLANT morphology; PLANTS
- Publication
Gene Conserve, 2011, Vol 10, Issue 41, p216
- ISSN
1808-1878
- Publication type
Article