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- Title
SARS-CoV-2-Specific Antibody Detection for Seroepidemiology: A Multiplex Analysis Approach Accounting for Accurate Seroprevalence.
- Authors
den Hartog, Gerco; Schepp, Rutger M; Kuijer, Marjan; GeurtsvanKessel, Corine; van Beek, Josine; Rots, Nynke; Koopmans, Marion P G; van der Klis, Fiona R M; van Binnendijk, Robert S
- Abstract
<bold>Background: </bold>The COVID-19 pandemic necessitates better understanding of the kinetics of antibody production induced by infection with SARS-CoV-2. We aimed to develop a high-throughput multiplex assay to detect antibodies to SARS-CoV-2 to assess immunity to the virus in the general population.<bold>Methods: </bold>Spike protein subunits S1 and receptor binding domain, and nucleoprotein were coupled to microspheres. Sera collected before emergence of SARS-CoV-2 (n = 224) and of non-SARS-CoV-2 influenza-like illness (n = 184), and laboratory-confirmed cases of SARS-CoV-2 infection (n = 115) with various severities of COVID-19 were tested for SARS-CoV-2-specific IgG concentrations.<bold>Results: </bold>Our assay discriminated SARS-CoV-2-induced antibodies and those induced by other viruses. The assay specificity was 95.1%-99.0% with sensitivity 83.6%-95.7%. By merging the test results for all 3 antigens a specificity of 100% was achieved with a sensitivity of at least 90%. Hospitalized COVID-19 patients developed higher IgG concentrations and the rate of IgG production increased faster compared to nonhospitalized cases.<bold>Conclusions: </bold>The bead-based serological assay for quantitation of SARS-CoV-2-specific antibodies proved to be robust and can be conducted in many laboratories. We demonstrated that testing of antibodies against multiple antigens increases sensitivity and specificity compared to single-antigen-specific IgG determination.
- Subjects
NETHERLANDS; VIRAL pneumonia; RESEARCH; IMMUNOGLOBULINS; NUCLEAR proteins; CLASSIFICATION; RESEARCH methodology; COVID-19; CASE-control method; PHARMACOKINETICS; PATIENTS; EVALUATION research; MEDICAL cooperation; IMMUNOASSAY; COMPARATIVE studies; EPIDEMICS; IMMUNITY; VIRAL antibodies; MEMBRANE proteins; RECEIVER operating characteristic curves; EPIDEMIOLOGICAL research
- Publication
Journal of Infectious Diseases, 2020, Vol 222, Issue 7, p1452
- ISSN
0022-1899
- Publication type
journal article
- DOI
10.1093/infdis/jiaa479