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- Title
Integration of whole transcriptome spatial profiling with protein markers.
- Authors
Ben-Chetrit, Nir; Niu, Xiang; Swett, Ariel D.; Sotelo, Jesus; Jiao, Maria S.; Stewart, Caitlin M.; Potenski, Catherine; Mielinis, Paulius; Roelli, Patrick; Stoeckius, Marlon; Landau, Dan A.
- Abstract
Spatial transcriptomics and proteomics provide complementary information that independently transformed our understanding of complex biological processes. However, experimental integration of these modalities is limited. To overcome this, we developed Spatial PrOtein and Transcriptome Sequencing (SPOTS) for high-throughput simultaneous spatial transcriptomics and protein profiling. Compared with unimodal measurements, SPOTS substantially improves signal resolution and cell clustering and enhances the discovery power in differential gene expression analysis across tissue regions. Visium spatial transcriptomics is combined with markers for more than 30 proteins.
- Publication
Nature Biotechnology, 2023, Vol 41, Issue 6, p788
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/s41587-022-01536-3