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- Title
Förster resonance energy transfer demonstrates a flavonoid metabolon in living plant cells that displays competitive interactions between enzymes
- Authors
Crosby, Kevin C.; Pietraszewska-Bogiel, Anna; Gadella, Theodorus W.J.; Winkel, Brenda S.J.
- Abstract
Abstract: We have used Förster resonance energy transfer detected by fluorescence lifetime imaging microscopy (FLIM-FRET) to provide the first evidence from living plants cells for the existence of a flavonoid metabolon. The distribution of flux within this system may be regulated by the direct competition of enzymes that catalyze key branch-point reactions, flavonol synthase 1 and dihydroflavonol 4-reductase, for association with the entry-point enzyme, chalcone synthase. Because the flavonoid enzymes were likely recruited from pathways of primary metabolism, our findings suggest a new general working model for the regulation of dynamic pathways in their native cellular context. Structured summary of protein interactions: CHS and FLS1 physically interact by fluorescence resonance energy transfer (View interaction) CHS and DFR physically interact by fluorescence resonance energy transfer (View Interaction 1, 2) FLS1 physically interacts with CHS and DFR by competition binding (View interaction)
- Subjects
PLANT cells &; tissues; RESONANCE; ENERGY transfer; FLAVONOIDS; ANTHOCYANIDINS; FLUORESCENCE microscopy; MULTIENZYME complexes; ARABIDOPSIS
- Publication
FEBS Letters, 2011, Vol 585, Issue 14, p2193
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2011.05.066