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- Title
Nepmucin/CLM-9, an Ig domain-containing sialomucin in vascular endothelial cells, promotes lymphocyte transendothelial migration in vitro
- Authors
Jin, Soojung; Umemoto, Eiji; Tanaka, Toshiyuki; Shimomura, Yoshimitsu; Tohya, Kazuo; Kunizawa, Keiji; Yang, Bo-Gie; Jang, Myoung Ho; Hirata, Takako; Miyasaka, Masayuki
- Abstract
Abstract: Nepmucin/CLM-9 is an Ig domain-containing sialomucin expressed in vascular endothelial cells. Here we show that, like CD31, nepmucin was localized to interendothelial contacts and to vesicle-like structures along the cell border and underwent intracellular recycling. Functional analyses showed that nepmucin mediated homotypic and heterotypic cell adhesion via its Ig domain. Nepmucin-expressing endothelial cells showed enhanced lymphocyte transendothelial migration (TEM), which was abrogated by anti-nepmucin mAbs that block either homophilic or heterophilic binding. Notably, the mAbs that inhibited homophilic binding blocked TEM without affecting lymphocyte adhesion. These results suggest that endothelial nepmucin promotes lymphocyte TEM using multiple adhesion pathways.
- Subjects
VASCULAR endothelial growth factors; VASCULAR endothelium; CELL adhesion; CELL migration; LYMPHOCYTES
- Publication
FEBS Letters, 2008, Vol 582, Issue 20, p3018
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.07.041