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- Title
Spectroelectrochemistry of cytochrome b559 in the D1–D2–Cyt b559 complex from spinach
- Authors
Shibamoto, Tadao; Kato, Yuki; Watanabe, Tadashi
- Abstract
Abstract: The redox potential of cytochrome b559 (Cyt b559) in the D1–D2–Cyt b559 complex from spinach has been determined to be +90±2mV vs. SHE at pH 6.0, by thin-layer cell spectroelectrochemistry for the first time. The redox potential, corresponding uniquely to the so-called “low-potential form”, exhibited a sigmoidal pH-dependence from pH 4.0 to 9.0, ranging from +115 to +50mV. An analysis of the pH-dependence based on model equations suggests that two histidine residues coordinating to the heme iron in the protein subunits may exert electrostatic influence on the redox potential of Cyt b559.
- Subjects
CYTOCHROMES; SPINACH; HEME; OXIDATION-reduction reaction
- Publication
FEBS Letters, 2008, Vol 582, Issue 10, p1490
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2008.03.041