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- Title
Properties of recombinant glycine decarboxylase P- and H-protein subunits from the cyanobacterium Synechocystis sp. strain PCC 6803
- Authors
Hasse, Dirk; Mikkat, Stefan; Thrun, Hans-Albrecht; Hagemann, Martin; Bauwe, Hermann
- Abstract
Abstract: The multi-enzyme complex glycine decarboxylase is important for one-carbon metabolism, essential for the photorespiratory glycolate cycle of plants, and comprises four different polypeptides, P-, H-, T-, and L-protein. We report on the production and properties of recombinant P-protein from the cyanobacterium Synechocystis and also describe features of recombinant H-protein from the same organism. The P-protein shows enzymatic activity with lipoylated H-protein and very low activity with H-apoprotein or lipoate as artificial cofactors. Its affinity towards glycine is unaffected by the presence and nature of the methyleneamine acceptor molecule. The cyanobacterial H-protein apparently forms stable dimers.
- Subjects
ACETIC acid; GLYCINE; AMINO acids; BIOCHEMISTRY
- Publication
FEBS Letters, 2007, Vol 581, Issue 7, p1297
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2007.02.037