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- Title
The complete set of tRNA species in Nanoarchaeum equitans
- Authors
Randau, Lennart; Pearson, Michael; Söll, Dieter
- Abstract
Abstract: The archaeal parasite Nanoarchaeum equitans was found to generate five tRNA species via a unique process requiring the assembly of seperate 5′ and 3′ tRNA halves [Randau, L., Münch, R., Hohn, M.J., Jahn, D. and Söll, D. (2005) Nanoarchaeum equitans creates functional tRNAs from separate genes for their 5′- and 3′-halves. Nature 433, 537–541]. Biochemical evidence was missing for one of the computationally-predicted, joined tRNAs designated as tRNATrp. Our RT-PCR and sequencing results identify this tRNA as tRNALys (CUU) joined at the alternative position between bases 30 and 31. We show that the intron-containing tRNATrp was misidentified in the initial Nanoarchaeum equitans genome annotation [E. Waters et al. (2003) The genome of Nanoarchaeum equitans: insights into early archaeal evolution and derived parasitism. Proc. Natl. Acad. Sci. USA 100, 12984–12988]. Along with a previously unidentified joined tRNAGln (UUG), Nanoarchaeum equitans exhibits 44 tRNAs and is enabled to read all 61 sense codons. Features unique to this set of tRNA molecules are discussed.
- Subjects
TRANSFER RNA; DNA polymerases; REVERSE transcriptase; GENOMES
- Publication
FEBS Letters, 2005, Vol 579, Issue 13, p2945
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.04.051