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- Title
The interaction of the hnRNP family member E1B-AP5 with p53
- Authors
Barral, Paola M.; Rusch, André; Turnell, Andrew S.; Gallimore, Phillip H.; Byrd, Philip J.; Dobner, Thomas; Grand, Roger J.A.
- Abstract
Abstract: Adenovirus early region 1B-associated protein 5, E1B-AP5, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family, was originally isolated on the basis of its ability to bind to the adenovirus 5 early region1B55K protein. Here, it has been demonstrated that E1B-AP5 interacts with mutant and wild-type p53 from human cells in pull-down assays using GST-E1B-AP5. This interaction has been confirmed by co-immunoprecipitation studies and pull-down experiments with in vitro translated E1B-AP5 and GST-p53. The binding site for E1B-AP5 has been mapped to the C-terminal region of p53. In reciprocal experiments, it has been shown that several regions of E1B-AP5 bound to p53 although it is probable that a major site of interaction is located between amino acids 395 and 732 of E1B-AP5. In reporter assays, E1B-AP5 inhibited p53 transcriptional activity although not as efficiently as the Ad5E1B55K protein. Transfection of E1B-AP5 into human tumour cells affected the cellular response to UV radiation, such that, although p53 expression was induced, little change in the level of p53-inducible genes could be observed.
- Subjects
ADENOVIRUSES; DNA viruses; VALUE-added tax; CANCER cells
- Publication
FEBS Letters, 2005, Vol 579, Issue 13, p2752
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.03.095