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- Title
Association of the Src homology 2 domain-containing leukocyte phosphoprotein of 76 kD (SLP-76) with the p85 subunit of phosphoinositide 3-kinase
- Authors
Shim, Eun Kyung; Moon, Chang Suk; Lee, Gi Yeon; Ha, Yun Jung; Chae, Suhn-Kee; Lee, Jong Ran
- Abstract
To investigate additional functions of the T cell adaptor, Src homology 2 (SH2) domain-containing leukocyte protein of 76 kD (SLP-76), we performed a yeast two-hybrid assay using the N-terminal region of SLP-76 fused with the kinase domain of Syk. By screening a human leukemia cDNA library, we identified the p85 subunit of phosphoinositide 3-kinase (PI3K) as one of the interacting molecules. Unlike the SH2 domain of Vav or Nck, tyrosine phosphorylation of SLP-76 at position 113 or 128 was sufficient for it to associate with the N-terminal SH2 of p85. Collectively, these data suggest that SLP-76 may play a role in PI3K signaling pathways.
- Subjects
HOMOLOGY (Biology); TYROSINE; PROTEINS; CELLS
- Publication
FEBS Letters, 2004, Vol 575, Issue 1-3, p35
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2004.07.090