We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Curcumin Ameliorates Hydrogen Peroxide-Induced Epithelial Barrier Disruption by Upregulating Heme Oxygenase-1 Expression in Human Intestinal Epithelial Cells.
- Authors
Wang, Na; Wang, Gai; Hao, JingXia; Ma, JunJi; Wang, Yan; Jiang, XiaoYu; Jiang, HuiQing
- Abstract
Background: Disruption of epithelial tight junctions (TJ) followed by loss of barrier function is of crucial importance in the pathogenesis of a variety of gastrointestinal disorders. Heme oxygenase-1 (HO-1), which can be induced by curcumin (Cur), provides protection against various forms of oxidative stress. Aims: The protective effect of Cur on oxidative stress-induced intestinal barrier disruption in human intestinal epithelial cells was elucidated in this study. Methods: HO-induced Caco-2 enterocytic monolayers were incubated in the presence or absence of Cur and/or zinc protoporphyrin (ZnPP). The trans-epithelial electrical resistance (TEER) and the flux of sodium fluorescein in the filter-grown Caco-2 cell monolayers were measured. The expression and localization of the TJ protein occludin and zonula occluden-1 (ZO-1) were evaluated by western blot and immunofluorescence microscopy. The mRNA and protein levels of HO-1 were analyzed by real-time PCR and western blot. Results: Cur attenuated HO-induced disruption of paracellular permeability (TEER 52.02 ± 10.15% vs 22.71 ± 3.11%; sodium fluorescein flux 12.41 ± 2.19% vs 32.00 ± 4.97%, P < 0.05) and induced HO-1 mRNA (6.64 ± 0.48 vs 3.22 ± 0.28, P < 0.05) and protein (291.00 ± 9.17% vs 99.00 ± 10.00%, P < 0.05) expression in Caco-2 cells. After administration of HO, occludin and ZO-1 proteins were restored by Cur (occludin 175.67 ± 29.50% vs 53.67 ± 24.19%, P < 0.05; ZO-1 139.67 ± 33.71% vs 36.00 ± 15.88%, P < 0.05) and this effect was blocked by HO-1 inhibitor, ZnPP (occludin 54.67 ± 10.02% vs 168.33 ± 36.47%, P < 0.05; ZO-1 50.00 ± 15.13% vs 117.67 ± 38.81%, P < 0.05). Conclusion: Cur protects human intestinal epithelial cells against HO-induced disruption of TJ and barrier dysfunction via the HO-1 pathway.
- Subjects
CURCUMIN; HYDROGEN peroxide; HEME oxygenase; GENE expression; EPITHELIAL cells; TIGHT junctions; OXIDATIVE stress
- Publication
Digestive Diseases & Sciences, 2012, Vol 57, Issue 7, p1792
- ISSN
0163-2116
- Publication type
Article
- DOI
10.1007/s10620-012-2094-7