We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
A synthetic CD4–heparan sulfate glycoconjugate inhibits CCR5 and CXCR4 HIV-1 attachment and entry.
- Authors
Baleux, Françoise; Loureiro-Morais, Latino; Hersant, Yael; Clayette, Pascal; Arenzana-Seisdedos, Fernando; Bonnaffé, David; Lortat-Jacob, Hugues
- Abstract
The HIV-1 envelope, gp120, which features the binding determinants for both CD4 and coreceptor recognition, is key for virus entry and represents an attractive pharmacological target. However, critical domains for entry (coreceptor and CD4 binding sites) are either cryptic or located in partially occluded cavities. Here we developed a chemical approach to synthesize a CD4-mimetic peptide linked to a heparan sulfate dodecasaccharide. This molecule binds to gp120, induces the exposure of the coreceptor binding domain and renders it available for interaction with the oligosaccharide. The linkage between the CD4 mimetic and the heparan sulfate derivative provides strong cooperative effects, resulting in low-nanomolar antiviral activity toward both CCR5- and CXCR4-tropic HIV-1 strains. This compound, which has the unique ability to simultaneously target two critical and highly conserved regions of gp120, establishes a new type of inhibitor and suggests a general concept for the inhibition of numerous other biological systems.
- Subjects
HIV virus enzymes; GLYCOCONJUGATES; SYNTHETIC biology; BIOLOGICAL systems; VIRUS inhibitors
- Publication
Nature Chemical Biology, 2009, Vol 5, Issue 10, p743
- ISSN
1552-4450
- Publication type
Article
- DOI
10.1038/nchembio.207