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- Title
Inhibition of Proliferation and Induction of Apoptosis in Human Renal Carcinoma Cells by Anti-telomerase Small Interfering RNAs.
- Authors
Jun-Nian Zheng; Ya-Feng Sun; Dong-Sheng Pei; Jun-Jie Liu; Jia-Cun Chen; Wang Li; Xiao-Qing Sun; Qi-Duo Shi; Rui-Fa Han; Teng-Xiang Ma
- Abstract
Telomerase is an attractive molecular target for cancer therapy because it is present in most malignant cells but is undetectable in most normal somatic cells. Human telomerase consists of two subunits, an RNA component (hTR) and a human telomerase reverse transcriptase component (hTERT). Small interfering RNA (siRNA), one kind of RNA interferences, has been demonstrated to be an effective method to inhibit target gene expression in human cells. We investigated the effects of siRNA targeting at both hTR and hTERT mRNA on the inhibition of telomerase activity in human renal carcinoma cells (HRCCs). The proliferation and apoptosis of HRCCs were examined. The treatment of HRCCs using hTR and hTERT siRNAs resulted in significant decrease of hTR mRNA, hTERT mRNA and hTERT protein. The siRNA can also inhibit the telomerase activity and the proliferation of HRCCs. Moreover, they can induce apoptotic cell death in a dose-dependent manner. From these findings, we propose that the inhibition of telomerase activity using siRNA targeting hTR and hTERT might be a rational approach in renal cancer therapy. Edited by Jian DING
- Subjects
TELOMERASE; CANCER treatment; SOMATIC cells; RNA; GENE expression; SMALL interfering RNA
- Publication
Acta Biochimica et Biophysica Sinica, 2006, Vol 38, Issue 7, p500
- ISSN
1672-9145
- Publication type
Article
- DOI
10.1111/j.1745-7270.2006.00182.x