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- Title
Long-lasting Chikungunya virus Neutralizing Antibodies in Convalescent patients´ sera from an endemic region of Mexico.
- Authors
Muñoz Herrera, J. C.; Suárez-Gómez, A. G.; Salinas-Trujano, J. R.; Pérez-Tapia, S. M.; Pedraza-Escalona, M. M.
- Abstract
Chikungunya fever (CF) is caused by CHIK virus and is transmitted by an arthropod vector. Currently, the climatic conditions spread the migration of the vector, increasing the possibilities of new outbreaks around the world. We evaluate the persistence of antibodies against CHIKV in a population of Guerrero by indirect ELISA, Western Blot (WB), and plaque reduction neutralization test (PRNT), nine years after the 2014 outbreak. To considerate positive specimens that recognize E2-CHIKV protein in ELISA, the ratio between positive and negative samples absorbance is ≥ 2, using a serum from one patient obtained during CHIKV outbreak of 2014 as a positive control. From thirty-four sera, the antibody titer was determined using a three-fold serial dilution, starting with 1:300. The samples were classified in four groups: 1) 16.6 % showed very high titer (≥10 001); 2) 8.3 % high titer (10 000-5 001); 3) 41.6 % intermediate titer (5 000-1 001); and 4) 33.3 % low titer (1 000-300). One sixth of these, showed neutralizing activity against the isolate Chikungunya virus 033. The protein E2 (37.5 kDa) was identify in almost all the samples by WB. These results indicate that long-lasting humoral immunity against CHIKV (due to the presence of long-live plasma cells) is reactivated by continuous exposure to the virus.
- Publication
Veterinaria México OA, 2024, Vol 11, p45
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305