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- Title
Further Characterization of CD34-Low/Negative Mouse Hematopoietic Stem Cells.
- Authors
NAKAUCHI, HIROMITSU; TAKANO, HINA; EMA, HIDEO; OSAWA, MASATAKE
- Abstract
A bstract: We have previously reported that in adult mouse bone marrow, CD34low/− c-kit+ Sca-1+ lineage markers negative (Lin−) (CD34−KSL) cells represent hematopoietic stem cells with long-term marrow repopulating ability whereas CD34+ c-kit+ Sca-1+ Lin− (CD34+KSL) cells are progenitors with short-term reconstitution capacity. To further characterize cells in those two populations, relative expression of various genes wereee examniend by reverse transcriptase polymerase chain reaction (RT-PCR). In CD34−KSL Cells, none of the genes sturied was found to be expressed with the exception of GATA-2, IL-1Rα, IL-2Rγ, AIC-2B, c-kit, EPO-R, and c-mpl. In contrast, expression of GATA-1 and all cytokine receptor genes examined except IL-2Rβ, IL-7Rα and IL-9Rα were found in CD34+KSL. The difference between these two populations was also shown in single cell culture analysis of these cells. when cells were clone sorted and cultured in the presence of SCF, IL-3 and EPO, CD34−KSL cells required much more time to undergo the first cell division than CD34+KSL cells. dormancy and random fashion of cell division by CD34−KSL cells were also evident by the analysis of the second cell division, which was found to be delayed and unsynchronous comapred with CD34+KSL cells. Clonal culture analusis showed that CD34−KSL cells were more potent in proliferation and multilineage differentiation capacities than CD34+KSL cells. In a paired-daughter cell experiment, 75% of CD34−KSL and 50% of CD34+KSL paired-daughter-derived colonies were nonidentical wiht wide vairety of lineage combinations. Taken together, these data support our previous notion that CD34−KSL cells are at higher rank in hematopoietic hierarchy than CD34+KSL cells. In addition, our results using highly enriched stem cell population directly obtained from mouse bone marrow support the proposed stochastic nature of lineage commitment.
- Publication
Annals of the New York Academy of Sciences, 1999, Vol 872, Issue 1, p57
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.1999.tb08453.x