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- Title
Hypoxia-Inducible Factor 1-Alpha Regulates Cancer-Inhibitory Effect of Human Mesenchymal Stem Cells.
- Authors
Hu, Yuan; Bai, Jing; Hou, Shu-Xun; Tang, Jin-Shu; Shi, Xiu-Xiu; Qin, Jiang; Ren, Neng
- Abstract
Mesenchymal stem cells (MSCs) have been shown to be able to inhibit cancer cells growth. In this study, we investigate the role and the molecular mechanism of hypoxia-inducible factor 1-alpha (HIF-1α) in inhibition of cancer cell proliferation by human MSCs through depletion and overexpression of HIF-1α in human MSCs. We found that the cell culture medium from HIF-1α-depleted Z3 cells significantly promotes breast cancer MCF-7 cell proliferation and colony formation. The expression of p21 is increased in MCF-7 cells, but p53 level remains unchanged. In contrast, the cultured medium from HIF-1α-overexpressed Z3 cells dramatically inhibits MCF-7 cell proliferation and colony formation. The expression of p21 is inhibited in MCF-7 cells, but p53 does not change. We conclude HIF-1α promotes inhibitory effect of human MCSs on breast cancer cell proliferation and colony formation. This process is tightly correlated with cell cycle protein p21 level in cancer cells.
- Subjects
HYPOXIA-inducible factor 1; MESENCHYMAL stem cells; CANCER cell growth regulation; CANCER cell proliferation; THERAPEUTICS
- Publication
Cell Biochemistry & Biophysics, 2015, Vol 72, Issue 1, p131
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-014-0420-8