We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Somatic embryogenesis and direct as well as indirect organogenesis in Lilium pumilum DC. Fisch., an endangered ornamental and medicinal plant.
- Authors
Zhang, Jing; Gai, MeiZhu; Li, XueYan; Li, TianLai; Sun, HongMei
- Abstract
Somatic embryogenesis and organogenesis inLilium pumilumwere successfully regulated by picloram, ?-naphthaleneacetic acid (NAA), and 6-benzyladenine (BA). In organogenesis, the highest shoot regeneration frequency (92.5%) was obtained directly from bulb scales on Murashige and Skoog (MS) medium containing 2.0 mg L?1BA and 0.2 mg L?1NAA, while organogenic callus (OC) formed from leaves on MS medium supplemented with 1.0 mg L?1BA and 0.5 mg L?1NAA. Following subculture, 76.7% of OC regenerated shoots. In somatic embryogenesis, the combination of picloram and NAA increased the amount of embryogenic callus (EC) that formed with a maximum on 90.7% of all explants which formed 11 somatic embryos (SEs) per explant. Differences between EC and OC in cellular morphology and cell differentiation fate were easily observed. SEs initially formed via an exogenous or an endogenous origin. The appearance of a protoderm in heart-shaped SE and the bipolar shoot–root development in oval-shaped SE indicated true somatic embryogenesis. This protocol provides a new and detailed regulation and histological examination of regeneration pattern inL. pumilum. Regulation and histological examination of somatic embryogenesis and organogenesis inLilium pumilum.
- Subjects
SOMATIC embryogenesis; LILIES; PLANT morphogenesis; PHYSIOLOGY
- Publication
Bioscience, Biotechnology & Biochemistry, 2016, Vol 80, Issue 10, p1898
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2016.1194178