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- Title
Association ofp53Gene Mutation with the Prognosis of Canine Lymphoid Tumors.
- Authors
Koshino, A.; Setoguchi, A.; Ohno, K.; Tsujimoto, H.
- Abstract
Many dogs with lymphoid tumors develop resistance to chemotherapy. As a mechanism of drug resistance in canine lymphoma, ATP-dependent drug efflux by P-glycoprotein was reported, however, inhibition of apoptosis mediated by P53 inactivation has not been investigated. In this study, we investigated the relationship betweenp53gene mutation and clinical drug resistance in canine lymphoid tumors.Tumor specimens were obtained from 44 dogs with lymphoid tumors. Mutations ofp53gene at exon 4–8 of these tumor tissues were examined by PCR-SSCP(single strand conformational polymorphism) analysis, followed by nucleotide sequencing of the abnormal bands. The cases were treated with UW-Madison protocol, and its response was evaluated by the tumor size or the number of peripheral leukemic cells.Of the 44 dogs, 15 dogs(34%) hadp53mutation, whereas 29 dogs(66%) were devoid ofp53mutation, before or during the chemotherapeutic protocol. Rate of good response(CR and PR) to chemotherapy was significantly lower in the dogs withp53mutation(20%) than those withoutp53mutation(55%)(p = 0.022). Median overall survival duration after examination ofp53mutation was significantly shorter in dogs withp53mutation(101days) than those withoutp53mutation(223days)(p = 0.008).Lymphoid tumors withp53mutations were shown to have worse prognosis than those withoutp53mutation.
- Subjects
LABORATORY dogs; DRUG therapy; P-glycoprotein; GLYCOPROTEINS; TUMORS; NUCLEOTIDES
- Publication
Veterinary & Comparative Oncology, 2005, Vol 3, Issue 1, p42
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/j.1476-5810.2005.0064p.x