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- Title
An Efficient One-Pot Four-Segment Condensation Method for Protein Chemical Synthesis.
- Authors
Shan Tang; Yan-Yan Si; Zhi-Peng Wang; Kun-Rong Mei; Xin Chen; Jing-Yuan Cheng; Ji-Shen Zheng; Lei Liu
- Abstract
Successive peptide ligation using a one-pot method can improve the efficiency of protein chemical synthesis. Although one-pot three-segment ligation has enjoyed widespread application, a robust method for one-pot four-segment ligation had to date remained undeveloped. Herein we report a new one-pot multisegment peptide ligation method that can be used to condense up tofour segments with operational simplicity and high efficiency. Its practicality is demonstrated by the one-pot four-segment synthesis of a plant protein, crambin, and a human chemokine, hCCL21.
- Subjects
PEPTIDE bonds; PROTEIN synthesis; LIGATION reactions; CHEMICAL synthesis; CONDENSATION
- Publication
Angewandte Chemie, 2015, Vol 127, Issue 19, p5805
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201500051