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- Title
Poly(3-hydroxybutyrate) synthesis in fed-batch culture of Ralstonia eutropha with phosphate limitation under different glucose concentrations.
- Authors
Longan Shang; Min Jiang; Ho Nam Chang
- Abstract
Poly(3-hydroxybutyrate) (PHB) was produced by fed-batch cultures of Ralstonia eutropha with phosphate limitation under different glucose concentrations. When glucose was kept at 2.5 g l-1, cell growth and PHB synthesis were limited due to the shortage of carbon source but a higher PHB content occurred in the cell-growth stage. This shows that a low glucose concentration is favorable for PHB accumulation in R. eutropha. PHB obtained with glucose at 9 g l-1 is 1.6 times that obtained with 40 g l-1. When glucose was in the range of 9 to 40 g l-1, PHB concentration and productivity decreased significantly with the increase of glucose concentration. The highest PHB productivity was obtained with glucose at 9 g l-1.
- Subjects
RALSTONIA; PSEUDOMONADACEAE; PHOSPHATES; GLUCOSE; CELL culture; SUCROSE
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 17, p1415
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1025047410699