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- Title
HLA-A0201 positive pancreatic cell lines: new findings and discrepancies.
- Authors
Kuichun Zhu; Lizee, Gregory; Cano, Pedro; Fernando-Vina, Marcelo; Baoan Ji; Abbruzzese, James; Hwu, Patrick; Radvanyi, Laszlo; Chang, David Z.
- Abstract
Pancreatic cancer is being pursued as an immunotherapy target using antigen-specific vaccine approaches activating CD8+ CTL and CD4+ T-helper cells. CD8+ CTL exert their anti-tumor effects in an HLA-restricted manner and only tumor cells carrying a matched HLA class I sub-type are targets for antigen-specific CTL. In the process of characterizing CD8+ T cell responses against pancreatic cancer, we screened a number of human pancreatic tumor cell lines for HLA-A0201 positive (HLA-A2+) cell lines to be used in the evaluation of CTL function. This analysis revealed some new findings and discrepancies in the literature on the HLA sub-type of some commonly used pancreatic cell lines. We found that Capan-1 cells, originally reported to be HLA-A0201+, actually only express HLA-A010101 and HLA-A300101 and were targets for HLA-A0201-restricted CTL only after transduction with an HLA-A0201-expressing lentivirus. Panc-1 cells were found to be HLA-A0201 positive, in agreement with published reports, while CF-Pac-1 cells were found to express both HLA-A020101 and HLA-A030101. We also found a normal human pancreatic ductal epithelial cell line, HPDE, to be HLA-A0201 positive. Our findings were verified with two different sequence-based typing methods, antibody staining followed by flow cytometry analysis, and functional analysis using an HLA-A0201-restricted peptide-specific T cell response.
- Subjects
PANCREATIC cancer; CANCER immunotherapy; CANCER vaccines; CANCER cells; EPITHELIAL cells; LENTIVIRUSES
- Publication
Cancer Immunology, Immunotherapy, 2007, Vol 56, Issue 5, p719
- ISSN
0340-7004
- Publication type
Article
- DOI
10.1007/s00262-006-0217-8