We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cleavage sites of the “P3 region” in the nonstructural polyprotein precursor of a dicistrovirus.
- Authors
Nakashima, Nobuhiko; Nakamura, Yuri
- Abstract
Plautia stali intestine virus (PSIV) is a member of the family Dicistroviridae. Dicistroviruses, like picornaviruses, are thought to encode a 3C-like protease; however, the cleavage sites of dicistroviral nonstructural polyprotein precursors are unknown except for those in genome-linked virus protein (VPg) regions. We expressed part of the PSIV polyprotein in Escherichia coli and detected autoprocessed viral proteins. N-terminal sequence analysis of the autoprocessed proteins showed that Q/GWLSW and Q/NGVFD in the PSIV sequence correspond to 2C/3A and 3C/3D cleavage sites in picornaviruses. Alignments of deduced amino acid sequences of the “P3 region” suggest that these cleavage sites can be predicted.
- Subjects
ESCHERICHIA coli; INTESTINAL diseases; PICORNAVIRUS infections; PICORNAVIRUSES; SCISSION (Chemistry); VIRAL proteins; AMINO acid sequence
- Publication
Archives of Virology, 2008, Vol 153, Issue 10, p1955
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-008-0208-5