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- Title
Characterization of complete genome sequence of the spring viremia of carp virus isolated from common carp ( Cyprinus carpio) in China.
- Authors
Teng, Y.; Liu, H.; Lv, J. Q.; Fan, W. H.; Zhang, Q. Y.; Qin, Q. W.
- Abstract
The complete genome of spring viraemia of carp virus (SVCV) strain A-1 isolated from cultured common carp ( Cyprinus carpio) in China was sequenced and characterized. Reverse transcription-polymerase chain reaction (RT-PCR) derived clones were constructed and the DNA was sequenced. It showed that the entire genome of SVCV A-1 consists of 11,100 nucleotide base pairs, the predicted size of the viral RNA of rhabdoviruses. However, the additional insertions in bp 4633–4676 and bp 4684–4724 of SVCV A-1 were different from the other two published SVCV complete genomes. Five open reading frames (ORFs) of SVCV A-1 were identified and further confirmed by RT-PCR and DNA sequencing of their respective RT-PCR products. The 5 structural proteins encoded by the viral RNA were ordered 3′-N-P-M-G-L-5′. This is the first report of a complete genome sequence of SVCV isolated from cultured carp in China. Phylogenetic analysis indicates that SVCV A-1 is closely related to the members of the genus Vesiculovirus, family Rhabdoviridae.
- Subjects
CHINA; CARP; PLANT viruses; DIAGNOSTIC use of polymerase chain reaction; NUCLEOTIDE sequence; RHABDOVIRUSES
- Publication
Archives of Virology, 2007, Vol 152, Issue 8, p1457
- ISSN
0304-8608
- Publication type
Article
- DOI
10.1007/s00705-007-0971-8