We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Overexpression of TAT-PTD-diapause hormone fusion protein in tobacco and its effect on the larval development of Helicoverpa armigera (Lepidoptera: Noctuidae).
- Authors
Zhou, Zhou; Li, Yongli; Yuan, Chunyan; Doucet, Daniel; Zhang, Yongan; Qu, Liangjian
- Abstract
BACKGROUND The diapause hormone (DH) has been shown either to induce or to terminate diapause, depending on the insect species. In a previous study we demonstrated that the DH from Clostera anastomosis (caDH) has biological activity in Helicoverpa armigera, which prompted us to examine the potential growth-inhibiting or antiherbivory effects of the TAT-caDH fusion protein when expressed in transgenic plants. RESULTS In this study, we produced transgenic tobacco plants expressing either the TAT-caDH protein or a TAT-caDH-eGFP fusion version that allowed tracking of the fluorescent protein in plant tissues. Our results indicate that H. armigera larvae feeding on transgenic tobacco expressing TAT-caDH exhibited a significantly reduced survival rate and weight gain. However, larvae feeding on transgenic tobacco expressing TAT-caDH-eGFP were unaffected. While fusion of the eGFP gene influenced the bioactivity of caDH in larvae, TAT-caDH-eGFP can still penetrate the insect midgut cell membrane. CONCLUSION TAT-caDH increases DH stability in oral delivery. Our results may help in targeting DH-dependent physiological processes in insects for improving herbivore tolerance in economically important crops. © 2016 Society of Chemical Industry
- Subjects
DIAPAUSE; HELICOVERPA armigera; TRANSGENIC plants; CHIMERIC proteins; LARVAE
- Publication
Pest Management Science, 2017, Vol 73, Issue 6, p1197
- ISSN
1526-498X
- Publication type
Article
- DOI
10.1002/ps.4443