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- Title
增强 miR-185-5p 表达可减轻小鼠实验性牙周炎的炎症反应 .
- Authors
李 昊; 叶枝茂; 罗诒财; 李孔梅; 麦昱颖; 王 琪
- Abstract
BACKGROUND: The pathogenesis of periodontitis is still unclear, and its therapeutic target remains to be explored. Recent studies have showed that miR-185- 5p has anti-inflammatory effects in many diseases. However, it is unclear whether miR-185-5p can influence the inflammatory response in periodontitis. OBJECTIVE: To investigate the effect of regulating miR-185-5p expression on inflammatory response in experimental periodontitis in mice. METHODS: Twenty-four C57B/6J mice were randomly divided into normal control group, periodontitis control group, periodontitis miR-NC group and periodontitis miR-mimics group (n=6 per group). Except for the normal control group, other groups were induced into experimental periodontitis. Four weeks after modeling, in the periodontitis miR-mimics group, 100 μL of normal saline containing 0.5 mmol/L miR-185-5p mimics was injected into the proximal and distal gingival papillae of bilateral mandibular second molars of mice. In the periodontitis miR-NC group, 100 μL of normal saline containing 0.5 mmol/L negative control miR-NC was injected. In the normal control group and the periodontitis control group, the same amount of normal saline was injected. After 8 weeks of administration, all mice were sacrificed. Micro-CT was used to detect the left mandibles, and qRT-PCR was used to detect the gene expression of miR-185-5p, marginal zone B and B-1 cell-specific protein (MZB1), tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) in the gingiva of left mandibles. Immunohistochemical staining was used to detect the protein expression of MZB1, phospho-p65 NF-κB (pp65), TNF-α and IL-6 in the gingiva of right mandibles. RESULTS AND CONCLUSION: Micro-CT examination results showed that compared with the periodontitis control group and periodontitis miR-NC group, alveolar bone loss in the periodontitis miR-mimics group was decreased (P < 0.05). qRT-PCR results showed that the expression of miR-185-5p in the periodontitis miR-mimics group was higher than that in the normal control group, periodontitis control group, and periodontitis miR-NC group (P < 0.05). Compared with the periodontitis control group and periodontitis miR-NC group, the mRNA expression of MZB1, TNF-α, and IL-6 was decreased in the periodontitis miR-mimics group (P < 0.05). Immunohistochemical staining results showed that compared with the periodontitis control group and periodontitis miR-NC group, the protein expression of MZB1, pp65, TNF-α and IL-6 was decreased in the periodontitis miR-mimics group (P < 0.05). Therefore, increasing the expression of miR-185-5p can inhibit the inflammatory response in periodontitis and the resorption of alveolar bone, which may be associated with the suppression of MZB1 and nuclear fctor-κB pathway in the gingival tissue.
- Subjects
BONE resorption; TUMOR necrosis factors; ALVEOLAR process; IMMUNOSTAINING; X-ray computed microtomography
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2023, Vol 27, Issue 35, p5676
- ISSN
2095-4344
- Publication type
Article
- DOI
10.12307/2023.892