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- Title
Hypergravity Effects on the Retina and Intraocular Pressure in Mice.
- Authors
Young Jun Kim; JaeSeung Chung; Tae Young Jang; Young Hyo Kim; HeeSeung Chin
- Abstract
BACKGROUND: We investigated the effect of exposure to+10.0 G for 4 h on the intraocular pressure and the retina of mice. METHODS: We exposed 10 mice to +10.0 Gz for 4 h by using a centrifugal acceleration test facility for animals. Intraocular changes were compared before and after hypergravity exposure. The eyeballs of the mice were enucleated after measuring the intraocular pressure. Tissue slides of the retina were prepared with hematoxylin and eosin (H&E) for histological examination and immunohistochemical analyses for vascular endothelial growth factor-A (VEGF-A), VEGF receptor 1 (VEGF-R1), VEGF-R2, glial fibrillary acidic protein (GFAP), and glutamine synthetase (GS). RESULTS: The average intraocular pressure was 7.7 ± 0.86 mmHg before the hypergravity exposure and 6.65 ± 0.67 mmHg after the exposure. No histological difference was observed between the retinas in the two groups. The levels ofVEGF-A, VEGF-R1, VEGF-R2, GFAP, and GS as assessed by immunohistochemistry were increased in the group exposed to hypergravity compared to the control group. DISCUSSION: Repeated exposure to a high level of hypergravity could cause elevation of intraocular pressure and hypoxic damage to the retina.
- Subjects
INTRAOCULAR pressure; OCULAR hypertension; VASCULAR endothelial growth factors; GLIAL fibrillary acidic protein; GLUTAMINE synthetase
- Publication
Aerospace Medicine & Human Performance, 2016, Vol 87, Issue 1, p13
- ISSN
2375-6314
- Publication type
Article
- DOI
10.3357/AMHP.4213.2016