We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Apoptotic and Autophagic Cell Death Effects of the Hexane Extract of Tropical Marine Algae Halymenia durvillei against Human Glioblastoma Cells: In vitro and in silico Studies.
- Authors
Vivithanaporn, Pornpun; Siangcham, Tanapan; Tanawoot, Varitta; Settacomkul, Rapeewan; Pranweerapaiboon, Kanta; Meemon, Krai; Niamnont, Nakorn; Tamtin, Montakan; Sobhon, Prasert; Sangpairoj, Kant
- Abstract
Glioblastoma (GBM) considered as aggressive brain cancer with high mortality rate in patients even after surgical resection. Resistant to chemotherapy is the major problem in GBM therapy. Discovery of novel bioactive compounds from algae is being investigated as alternative sources for potential treatment as well as prevention in glioblastoma. This study revealed the effects of marine red algae extract from hexane solvent fraction of Halymenia durvillei (HDHE) on proliferation and cell death in A172 human GBM cells. HDHE decreased proliferation and promoted cell cycle arrest at G2/M phase. HDHE induced apoptotic cell death in A172 cells through mitochondrial membrane dysfunction, the decrease of antiapoptotic Bcl-2 protein expression, and activation of caspase 3/7. Moreover, HDHE increased intracellular reactive oxygen species (ROS) production and accumulation of LC3-II, an autophagic marker. The docked conformation of palmitic acid, a major component of HDHE, showed a high affinity binding to TP53 and Beclin-1 as cell death-related target molecules. This research conclusively demonstrated that HDHE might serve as a potent anticancer agent against glioblastoma by promoting apoptotic and autophagic cell death in A172 human GBM cells.
- Subjects
MARINE algae; CELL death; GLIOBLASTOMA multiforme; PALMITIC acid; HEXANE; IN vitro studies; CELL cycle
- Publication
Trends in Sciences, 2024, Vol 21, Issue 2, p1
- ISSN
2774-0226
- Publication type
Article
- DOI
10.48048/tis.2024.7157