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- Title
İskemi/Reperfüzyon Hasarında Hesperidinin Nöroprotektif Etkisi.
- Authors
Çeliközlü, Saadet; Özyiğit, Filiz; Altıkat, Said; Köktürk, Sibel; Çeliközlü, Halit
- Abstract
Introduction: Hesperidin is a plant flavonoid with powerful antioxidant properties. With this feature, hesperidin was thought to be an effective agent in the prevention of ischemic damage. The aim of this study was to investigate the protective effect of different doses of hesperidin against cerebral ischemia/reperfusion injury. Materials and Methods: In this study, 5 groups were prepared as control, sham, ischemia/reperfusion (I/R), hesperidin50 (Hes 50), and hesperidin100 (Hes 100). 40 male Sprague-Dawley rats, 8 in each group, were used. Four vessel occlusion model was used to induce ischemia. 30minutes of ischemia and 30minutes of reperfusion were applied. Hesperidin was injected intraperitoneally 30minutes before ischemia. Hematoxylin eosin staining were performed, SOD, CAT, MDA, and total protein levels were determined in brain tissue. As a molecular marker, TNF-a mRNA expression levels were measured by RT-qPCR technique. Results: In the I/R group, CAT and SOD values decreased, the MDA value increased, a slight increase in the total protein value was found compared to the control group. CAT, SOD values increased, MDA and total protein values decreased significantly in Hes 50 and Hes 100 groups. A significant loss of neurons was observed in the CA1 region of the hippocampus in the I/R group compared to the control group. It was found that neuron damage in the hippocampus decreased and the number of neurons increased statistically significantly in the Hes 50 group compared to the I/R group. TNF-a mRNA expression values in brain tissue were significantly higher in the I/R group than control and sham groups. A significant decrease in the amount of mRNA expression was observed in the Hes50 group compared to the I/R group (p<0.05). Conclusion: According to the results of this study, hesperidin, which has antioxidant potential, showed neuroprotective and anti-inflammatory effects against oxidative stress damage caused by cerebral ischemia/reperfusion. While anti-inflammatory and neuroprotective effects were prominent in the low-dose hesperidin (Hes 50) group, the antioxidant effect was more dominant in boThHes 50 and Hes 100 groups.
- Publication
Sakarya Tıp Dergisi, 2023, Vol 13, Issue 2, p265
- ISSN
2146-2585
- Publication type
Article
- DOI
10.31832/smj.1182213