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- Title
SIRT3在氧糖剥夺再灌注损伤小鼠神经元中的表达及意义.
- Authors
王元欣; 张磊; 岳康异; 黑悦; 鱼洋; 武秀权; 蒋晓帆
- Abstract
Objective: To verify the expression and significance of silent information regulator 3 (SIRT3) in cerebral ischaemia using the oxygen glucose deprivation/reperfusion (OGD/R) model in vitro. Methods: Primary cultured cortical neurons from C57BL/6J exposed to oxygen-glucose deprivation for 2 h, 4 h, 6 h, 8h, and followed by 24 h of reperfusion. Additionally, the cell viability was detected by Cell Counting Kit-8 (CCK-8) assay, the cytotoxicity was measured by LDH kit assay, the expression of LC3-II, cleaved caspase-3 and SIRT3 was determined by western blot and the levels of LC3-II and SIRT3 were further asssayed by immunofluorescence. Results: The results showed that there was a time-dependent increase of LDH release and decrease of cell viability (P〈0.01). The results of Western blot demonstrated that LC3-II and SIRT3 increased and peaked at OGD 4h while the expression of Cleaved caspase-3 was elevated all the time. The results of immunofluorescence indicated that LC3-II and SIRT3 were significantly expressed in somas and neurites compared with the control. Conclusion: We observed that the expression of LC3 and SIRT3 changes in a similar pattern through a OGD/R model. Hence, SIRT3 might protects neurons against ischemic injury via regulating autophagy.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 5, p817
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.05.004