We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Discrete Typing Units of Trypanosoma cruzi Identified by Real-Time PCR in Peripheral Blood and Dejections of Triatoma infestans Used in Xenodiagnosis Descriptive Study.
- Authors
Zulantay, Inés; Muñoz, Gabriela; Liempi, Daniela; Rozas, Tamara; Manneschi, María José; Muñoz-San Martín, Catalina; Botto-Mahan, Carezza; Apt, Werner; Cabrera, Gonzalo
- Abstract
Chagas disease (ChD) is a vector zoonosis native to the American continent caused by the protozoan parasite Trypanosoma cruzi; the biological vectors are multiple species of hematophagous insects of the family Triatominae. A relevant aspect in the host–parasite relationship is the identification of the various genotypes of T. cruzi called discrete typing units (DTU) that circulate in mammals and vectors. In Chile, it has been described that the DTUs TcI, TcII, TcV, and TcVI circulate in infected humans, vectors, and wild animals. Identifying DTUs has acquired clinical importance, since it has been suggested that different genotypes could cause distinct pathologies, circulate in different geographical areas, and present different sensitivities to trypanocidal drugs. In this study, circulating T. cruzi DTUs in peripheral blood and Triatoma infestans dejections used in xenodiagnosis (XD) were amplified by qPCR in 14 Chilean patients with chronic ChD from highly endemic areas. More positive samples were detected by XD compared to peripheral blood samples, and 64.28% of the cases were simple infections and 35.72% mixed, with a statistically significant difference in the frequency of TcV DTU. This study would suggest that T. infestans from Chile is more competent to amplify one DTU over others, probably due to a process of co-evolution.
- Subjects
CHILE; TRIATOMA; TRYPANOSOMA cruzi; BLOODSUCKING insects; SADNESS; CHAGAS' disease; HOST-parasite relationships
- Publication
Pathogens, 2022, Vol 11, Issue 7, pN.PAG
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens11070787