We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Efficient generation of gene-modified pigs via injection of zygote with Cas9/sgRNA.
- Authors
Wang, Yong; Du, Yinan; Shen, Bin; Zhou, Xiaoyang; Li, Jian; Liu, Yu; Wang, Jianying; Zhou, Jiankui; Hu, Bian; Kang, Nannan; Gao, Jimin; Yu, Liqing; Huang, Xingxu; Wei, Hong
- Abstract
Co-injection of zygotes with Cas9 mRNA and sgRNA has been proven to be an efficient gene-editing strategy for genome modification of different species. Genetic engineering in pigs holds a great promise in biomedical research. By co-injection of one-cell stage embryos with Cas9 mRNA and Npc1l1 sgRNA, we achieved precise Npc1l1 targeting in Chinese Bama miniature pigs at the efficiency as high as 100%. Meanwhile, we carefully analyzed the Npc1l1 sgRNA:Cas9-mediated on- and off-target mutations in various somatic tissues and ovaries, and demonstrated that injection of zygotes with Cas9 mRNA and sgRNA is an efficient and reliable approach for generation of gene-modified pigs.
- Subjects
ZYGOTES; SWINE; MESSENGER RNA; TRANSFER-messenger RNA; RNA
- Publication
Scientific Reports, 2015, p8256
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep08256