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- Title
Reduced growth and proliferation dynamics of nasal epithelial stem/ progenitor cells in nasal polyps in vitro.
- Authors
Xue Min Yu; Chun Wei Li; Siew Shuen Chao; Ying Ying Li; Yan Yan; Xue Ning Zhao; Feng Gang Yu; Jing Liu; Liang Shen; Xin Liang Pan; Li Shi; De Yun Wang
- Abstract
Basal cells in nasal epithelium have stemness/progenitor characters and play essential roles in the epithelial remodeling in nasal polyps (NP). We investigate whether the human nasal epithelial stem/ progenitor cells (hNESPCs) from patients with NP are inherently distinct from those obtained from healthy controls. Epithelial basal cells were isolated and cultured for four passages from NP tissues and control nasal mucosa. hNESPCs from controls were stained positively with stem cell marker p63 and KRT5 and presented a consistent high Ki67 expression level over four passages. In contrast, hNESPCs from NP patients showed: i). a reduced growth and proliferation rate at each passage by evaluating colony-forming efficiency and doubling time; ii). a lower percentage of Ki67+ cells among p63+ cells in the colonies in late passages, which was also confirmed by immunostaining in the NP tissues. Thus reduced growth/proliferation dynamics in hNESPCs from NP could be an important pathological phenomenon in NP development.
- Subjects
NASAL polyps; PROGENITOR cells; POLYPS; STEM cells; EPITHELIAL cells; CELL proliferation
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep04619