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- Title
The Drosophila melanogaster sir2[sup +] Gene Is Nonessential and Has Only Minor Effects on Position-Effect Variegation.
- Authors
Åström, Stefan U.; Cline, Thomas W.; Rine, Jasper
- Abstract
Five Drosophila melanogaster genes belong to the highly conserved sir2 family, which encodes NAD[sup +]-dependent protein deacetylases. Of these five, dsir2[sup +] (CG5216) is most similar to the Saccharomyces cerevisiae SIR2 gene, which has profound effects on chromatin structure and life span. Four independent Drosophila strains were found with P-element insertions near the dsir2 transcriptional start site as well as extraneous linked recessive lethal mutations. Imprecise excision of one of these Pelements (PlacW07223) from a chromosome freed of extraneous lethal mutations produced dsir2[sup 17], a null intragenic deletion allele that generates no DSIR2 protein. Contrary to expectations from the report by Rosenberg and Parkhurst on their P-mobilization allele dSir2[sup ex10] homozygosity for dsir2[sup 17] had no apparent deleterious effects on viability, developmental rate, or sex ratio, and it fully complemented sir2[sup ex10]. Moreover, through a genetic test, we ruled out the reported effect of dSir2[sup ex10] on Sex-lethal expression. We did observe a modest, strictly recessive suppression of white[sup m4] position-effect variegation and a shortening of life span in dsir2 homozygous mutants, suggesting that dsir2 has some functions in common with yeast SIR2.
- Subjects
DROSOPHILA melanogaster; DROSOPHILA genetics; GENETIC transcription
- Publication
Genetics, 2003, Vol 163, Issue 3, p931
- ISSN
0016-6731
- Publication type
Article
- DOI
10.1093/genetics/163.3.931