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- Title
Association between Pro12Ala polymorphism of the PPAR-γ2 gene and insulin sensitivity in Brazilian patients with type-2 diabetes mellitus.
- Authors
Tavares, V.; Hirata, R. D. C.; Rodrigues, A. C.; Monte, O.; Salles, J. E. N.; Scalissi, N.; Speranza, A. C.; Hirata, M. H.
- Abstract
Peroxisome-proliferator-activated receptor-γ2 (PPAR-γ2) is a nuclear receptor that plays an important role in lipid metabolism and insulin sensitivity. The purpose of this study is to investigate the association of Pro12Ala polymorphism at the PPAR-γ2 gene in Brazilian patients with type-2 diabetes mellitus (T2D m) and controls (CG). Genomic DNA was obtained from 207 unrelated white people presenting with T2D m and from 170 controls. Anthropometric data included body mass index and waist to hip ratio. Biochemical parameters included fasting plasma glucose, total cholesterol, high- and low-density lipoprotein cholesterol, triglycerides, glycated haemoglobin and insulin. Systolic and diastolic blood pressures were also measured. Screening for mutations in the entire coding region of the PPAR-γ gene was performed by means of polymerase chain reaction (PCR), single-strand conformational polymorphism and sequencing. Pro12Ala polymorphism was analysed by using PCR-RFLP (restriction fragment-length polymorphism). One base substitution was identified – a C to G substitution in exon B of the PPAR-γ2 gene. The frequency of the Ala12 allele in T2D m (0.09) was similar to that found in CG (0.06, p = 0.185). In the T2D m group, Ala12 allele was associated with lower fasting plasma insulin levels (p = 0.036) and higher insulin sensitivity (p = 0.049) by means of homeostasis model assessment. Among obese people, there was no association between any of the T2D m or obesity-related traits and the Pro12Ala polymorphism. The results of our study suggest that people with the Ala12 allele of the PPAR-γ2 gene could be more sensitive to insulin than those carriers of the Pro12 allele among Brazilian Caucasians.
- Subjects
PEROXISOMES; LIPID metabolism; INSULIN; GENETIC polymorphisms; TYPE 2 diabetes; LIPOPROTEINS
- Publication
Diabetes, Obesity & Metabolism, 2005, Vol 7, Issue 5, p605
- ISSN
1462-8902
- Publication type
Article
- DOI
10.1111/j.1463-1326.2004.00453.x