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- Title
Overexpression of CD39 protects in a mouse model of preeclampsia.
- Authors
McRae, Jennifer L; Russell, Prudence A; Chia, Joanne SJ; Dwyer, Karen M
- Abstract
CD39 ( NTPDase1), a critical immune and vascular ecto-nucleotidase, hydrolyses pro-inflammatory and pro-thrombotic nucleotides (adenosine-5′-triphosphate ( ATP) and adenosine diphosphate) to adenosine. In humans, CD39 is the dominant ecto-nucleotidase in placental trophoblastic tissues and modulates ATP-dependent trophoblastic functions. CD39 is an integral component of regulatory T cells ( Treg), which are central to immunological tolerance and maintenance of normal pregnancy. We examined the impact of CD39 overexpression in a mouse model of preeclampsia. Matings were performed between virginal BALB/c female (wild-type ( WT) or CD39 transgenic ( CD39TG)) and C57BL/6 male mice. On days 10 and 12 of pregnancy BALB/c Th1-polarized cells were injected. Systolic blood pressure ( SBP) was measured throughout pregnancy. Mice were sacrificed at day 15 of pregnancy. Following transfer of Th1-polarized cells, SBP of pregnant WT mice increased (118 ± 3 mmHg to 142 ± 5 mmHg). Although ultrastructural changes were evident in the kidney this was not accompanied by significant proteinuria. SBP remained unchanged (115 ± 2 mmHg to 114 ± 3 mmHg) in pregnant CD39TG mice without evidence of renal lesions. We conclude that gestational hypertension can be induced in mice following transfer of maternally derived Th1-polarized cells and that overexpression of CD39 is protective in this model.
- Subjects
NUCLEOTIDASES; ADENOSINE triphosphatase; T cells; IMMUNOLOGICAL tolerance; PREECLAMPSIA; LABORATORY mice
- Publication
Nephrology, 2013, Vol 18, Issue 5, p351
- ISSN
1320-5358
- Publication type
Article
- DOI
10.1111/nep.12058