We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Quantitative Trait Loci Mapping for Plant Height in Tobacco using Linkage and Association Mapping Methods.
- Authors
Lirui Cheng; Aiguo Yang; Caihong Jiang; Min Ren; Yu Zhang; Quanfu Feng; Shaomei Wang; Yongsheng Guan; Chenggang Luo
- Abstract
Plant height (PH) is one of the most important agronomic traits in tobacco (Nicotiana tabacum L.). To gain a better understanding of its genetic basis, the F2 and F2:3 populations derived from NC82 (P1) and Kang88 (P2) cross were developed. The 193 F2 individuals and 145 F2:3 lines were further used to identify quantitative trait loci (QTL) affecting to PH in tobacco. Two main quantitative trait loci (M-QTL), designated qPH-6 and qPH-12, were mapped on genetic linkage groups 6 and 12, respectively, using linkage mapping (LM). The QTL qPH-12 was identified as stably expressed in different tobacco generations as well as in various environments. To further verify the chromosomal interval harboring qPH-12, a total of 96 accessions were subsequently evaluated for PH at two different sites and genotyped by 46 markers evenly distributed on chromosome 12. We found a total of four significant marker-trait associations that were identified with three different SSR markers (P < 0.01) and could explain 11.7 to 14.8% of the phenotypic variation in PH. One marker, PT55174, showed a significant association with PH (P < 0.01) and explained 13.9 and 14.4% of the phenotypic variation at two different sites. Taken together, our results could provide a new avenue for investigating the QTL in tobacco by combining LM and association mapping (AM), and facilitating our understanding of the inheritance of PH for marker-assisted selection (MAS) in tobacco.
- Subjects
PLANT physiology; TOBACCO -- Harvesting; LINKAGE (Genetics); PLANT genetics; PLANT population genetics
- Publication
Crop Science, 2015, Vol 55, Issue 2, p641
- ISSN
0011-183X
- Publication type
Article
- DOI
10.2135/cropsci2014.05.0404