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- Title
Biocontrol properties of some agricultural waste extracts on three nematode species in in vitro and in vivo conditions.
- Authors
Roshan-Bakhsh, Ali; Pourjam, Ebrahim; Ayyari, Mahdi; Pedram, Majid
- Abstract
Summary: Extracts of nine agricultural wastes prepared with five different solvents were assessed for their potential nematicidal activity against three nematode species, Aphelenchus avenae , Meloidogyne incognita and Pratylenchus neglectus , in in vitro condition. The 50% v/v hydro-ethanolic extracts showed the highest performance for two tested plant wastes of cabbage leaves and faba bean pods. These two extracts were tested on nematodes in three different concentrations. The highest in vitro nematistatic activity was recorded for 3000 and 1500 ppm of cabbage leaf extracts by 100% paralysis of all three nematode species after 48 h, and the highest nematicidal activity was recorded for the above-mentioned extract by 25-100% mortality depending on nematode species and extract concentration. A 14-94% mortality was recorded for all three species of nematodes after treatment with faba bean pod hydro-ethanolic extract in in vitro conditions. Hatching inhibition and repellent activity of cabbage leaf and faba bean pod extracts were observed in P. neglectus and M. incognita. In vivo assays confirmed the in vitro results when both of the extracts showed moderate to high inhibition of nematode population development and nematode infection parameters on tomato root system in pot experiments.
- Subjects
NEMATOCIDES; AGRICULTURAL wastes; SOUTHERN root-knot nematode; FAVA bean; ROOT-knot nematodes; NEMATODE infections; EXTRACTS
- Publication
Nematology, 2019, Vol 21, Issue 8, p837
- ISSN
1388-5545
- Publication type
Article
- DOI
10.1163/15685411-00003258