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- Title
Secondary metabolites and their bioactivities from Paecilomyces gunnii YMF1.00003.
- Authors
Su-Su Li; Shuai-Ling Qu; Juan Xie; Dong Li; Pei-Ji Zhao
- Abstract
Four new polyketides (1-4) and seven known compounds (5-11) including three polyketides and four sterols were isolated from the fermented extracts of Paecilomyces gunnii YMF1.00003. The new chemical structures were determined through the analysis of the nuclear magnetic resonance and highresolution electrospray ionization mass spectrometry, and their configurations were subsequently confirmed by nuclear overhauser effect spectroscopy, the calculated electronic circular dichroism (ECD) spectra, and quantum chemical calculations of the NMR data (qcc NMR). Based on the results of pre-activity screening and compound structure target prediction, certain metabolites were assayed to evaluate their cytotoxic and protein kinase Ca inhibitory activities. Results indicated that 3ß-hydroxy-7a-methoxy-5a,6a-epoxy-8(14),22E-dienergosta (8) exhibited potent cytotoxic activity, with half-maximal inhibitory concentration values of 3.00 ± 0.27 to 15.69 ± 0.61 µM against five tumor cells, respectively. The new compound gunniiol A (1) showed weak cytotoxic activity at a concentration of 40 µM. At a concentration of 20 µg/mL, compounds 1, 6, and 7 exhibited protein kinase Ca inhibition by 43.63, 40.93, and 57.66%, respectively. This study is the first to report steroids demonstrating good cytotoxicity and polyketides exhibiting inhibitory activity against protein kinase Ca from the extracts of P. gunnii.
- Subjects
POLYKETIDES; METABOLITES; ELECTROSPRAY ionization mass spectrometry; PAECILOMYCES; OVERHAUSER effect (Nuclear physics); PROTEIN kinases
- Publication
Frontiers in Microbiology, 2024, p1
- ISSN
1664-302X
- Publication type
Article
- DOI
10.3389/fmicb.2024.1347601