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- Title
Physical interaction of apoptosis-inducing factor with DNA and RNA.
- Authors
Vahsen, N; Candé, C; Dupaigne, P; Giordanetto, F; Kroemer, R T; Herker, E; Scholz, S; Modjtahedi, N; Madeo, F; Le Cam, E; Kroemer, G
- Abstract
Apoptosis-inducing factor (AIF) is a mitochondrial flavoprotein, which upon apoptosis induction translocates to the nucleus where it interacts with DNA by virtue of positive charges clustered on the AIF surface. Here we show that the AIF interactome, as determined by mass spectroscopy, contains a large panel of ribonucleoproteins, which apparently bind to AIF through the RNA moiety. However, AIF is devoid of any detectable RNAse activity both in vitro and in vivo. Recombinant AIF can directly bind to DNA as well as to RNA. This binding can be visualized by electron microscopy, revealing that AIF can condense DNA, showing a preferential binding to single-stranded over double-stranded DNA. AIF also binds and aggregates single-stranded and structured RNA in vitro. Single-stranded poly A, poly G and poly C, as well double-stranded A/T and G/C RNA competed with DNA for AIF binding with a similar efficiency, thus corroborating a computer-calculated molecular model in which the binding site within AIF is the same for distinct nucleic acid species, without a clear sequence specificity. Among the preferred electron donors and acceptors of AIF, nicotine adenine dinucleotide phosphate (NADP) was particularly efficient in enhancing the generation of higher-order AIF/DNA and AIF/RNA complexes. Altogether, these data support a model in which a direct interaction of AIF contributes to the compaction of nucleic acids within apoptotic cells.Oncogene (2006) 25, 1763–1774. doi:10.1038/sj.onc.1209206; published online 7 November 2005
- Subjects
APOPTOSIS; DNA; RNA; FLAVOPROTEINS; NUCLEOPROTEINS; NUCLEIC acids
- Publication
Oncogene, 2006, Vol 25, Issue 12, p1763
- ISSN
0950-9232
- Publication type
Article
- DOI
10.1038/sj.onc.1209206