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- Title
Development of a DC-Biased AC-Stimulated Microfluidic Device for the Electrokinetic Separation of Bacterial and Yeast Cells.
- Authors
Nasir Ahamed, Nuzhet Nihaar; Mendiola-Escobedo, Carlos A.; Perez-Gonzalez, Victor H.; Lapizco-Encinas, Blanca H.
- Abstract
Electrokinetic (EK) microsystems, which are capable of performing separations without the need for labeling analytes, are a rapidly growing area in microfluidics. The present work demonstrated three distinct binary microbial separations, computationally modeled and experimentally performed, in an insulator-based EK (iEK) system stimulated by DC-biased AC potentials. The separations had an increasing order of difficulty. First, a separation between cells of two distinct domains (Escherichia coli and Saccharomyces cerevisiae) was demonstrated. The second separation was for cells from the same domain but different species (Bacillus subtilis and Bacillus cereus). The last separation included cells from two closely related microbial strains of the same domain and the same species (two distinct S. cerevisiae strains). For each separation, a novel computational model, employing a continuous spatial and temporal function for predicting the particle velocity, was used to predict the retention time ( t R , p ) of each cell type, which aided the experimentation. All three cases resulted in separation resolution values R s > 1.5 , indicating complete separation between the two cell species, with good reproducibility between the experimental repetitions (deviations < 6%) and good agreement (deviations < 18%) between the predicted t R , p and experimental ( t R , e ) retention time values. This study demonstrated the potential of DC-biased AC iEK systems for performing challenging microbial separations.
- Subjects
MICROFLUIDIC devices; BACTERIAL cells; BACILLUS subtilis; RF values (Chromatography); BACILLUS cereus; CELL separation
- Publication
Biosensors (2079-6374), 2024, Vol 14, Issue 5, p237
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios14050237