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- Title
Rapid On-Site Detection of Arboviruses by a Direct RT-qPCR Assay.
- Authors
Mhamadi, Moufid; Mencattelli, Giulia; Gaye, Alioune; Ndiaye, El Hadji; Sow, Aïssatou Aïcha; Faye, Martin; Ndione, Marie Henriette Dior; Diagne, Moussa Moïse; Mhamadi, Moundhir; Faye, Ousmane; Weidmann, Manfred; Faye, Oumar; Diallo, Mawlouth; Diagne, Cheikh Tidiane
- Abstract
Arthropod-borne diseases currently constitute a source of major health concerns worldwide. They account for about 50% of global infectious diseases and cause nearly 700,000 deaths every year. Their rapid increase and spread constitute a huge challenge for public health, highlighting the need for early detection during epidemics, to curtail the virus spread, and to enhance outbreak management. Here, we compared a standard quantitative polymerase chain reaction (RT-qPCR) and a direct RT-qPCR assay for the detection of Zika (ZIKV), Chikungunya (CHIKV), and Rift Valley Fever (RVFV) viruses from experimentally infected-mosquitoes. The direct RT-qPCR could be completed within 1.5 h and required 1 µL of viral supernatant from homogenized mosquito body pools. Results showed that the direct RT-qPCR can detect 85.71%, 89%, and 100% of CHIKV, RVFV, and ZIKV samples by direct amplifications compared to the standard method. The use of 1:10 diluted supernatant is suggested for CHIKV and RVFV direct RT-qPCR. Despite a slight drop in sensitivity for direct PCR, our technique is more affordable, less time-consuming, and provides a better option for qualitative field diagnosis during outbreak management. It represents an alternative when extraction and purification steps are not possible because of insufficient sample volume or biosecurity issues.
- Subjects
RIFT Valley fever; ARBOVIRUS diseases; ARBOVIRUSES; VIRAL transmission; POLYMERASE chain reaction; COMMUNICABLE diseases
- Publication
Biosensors (2079-6374), 2023, Vol 13, Issue 12, p1035
- ISSN
2079-6374
- Publication type
Article
- DOI
10.3390/bios13121035