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- Title
Synergistic bacteriolysis by bacteriophage ϕEF24C endolysin ORF9 and lantibiotic nisin and its application to pulsed-field gel electrophoretic analysis of Enterococcus faecalis.
- Authors
Takemura-Uchiyama, Iyo; Uchiyama, Jumpei; Satoh, Miho; Ujihara, Takako; Daibata, Masanori; Matsuzaki, Shigenobu
- Abstract
Genotyping by pulsed-field gel electrophoresis (PFGE) is recognized as one of the most useful methods for epidemiological studies of drug-resistant Enterococcus faecalis nosocomial outbreaks. As the bacteriolysis procedure is a critical step in PFGE, an accurate and reliable alternative bacteriolytic method to the conventional protocol would be useful. In this study, we examined the applicability of endolysin ORF9, derived from the E. faecalis phage ϕEF24C, and lantibiotic nisin in sample preparation for PFGE. The results show that the cooperative actions of nisin and ORF9 synergistically lysed E. faecalis, which was then amenable to PFGE analysis.
- Publication
Annals of Microbiology, 2013, Vol 63, Issue 3, p1209
- ISSN
1590-4261
- Publication type
Article
- DOI
10.1007/s13213-012-0556-y