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- Title
Co-translational binding of importins to nascent proteins.
- Authors
Seidel, Maximilian; Romanov, Natalie; Obarska-Kosinska, Agnieszka; Becker, Anja; Trevisan Doimo de Azevedo, Nayara; Provaznik, Jan; Nagaraja, Sankarshana R.; Landry, Jonathan J. M.; Benes, Vladimir; Beck, Martin
- Abstract
Various cellular quality control mechanisms support proteostasis. While, ribosome-associated chaperones prevent the misfolding of nascent chains during translation, importins were shown to prevent the aggregation of specific cargoes in a post-translational mechanism prior the import into the nucleoplasm. Here, we hypothesize that importins may already bind ribosome-associated cargo in a co-translational manner. We systematically measure the nascent chain association of all importins in Saccharomyces cerevisiae by selective ribosome profiling. We identify a subset of importins that bind to a wide range of nascent, often uncharacterized cargoes. This includes ribosomal proteins, chromatin remodelers and RNA binding proteins that are aggregation prone in the cytosol. We show that importins act consecutively with other ribosome-associated chaperones. Thus, the nuclear import system is directly intertwined with nascent chain folding and chaperoning. Importins are known to facilitate nucleocytoplasmic transport and cytoplasmic chaperoning of some proteins. Here, the authors uncover that these proteins also act as co-translational chaperones for specific sets of proteins, for example ribonucleic acid binding factors.
- Subjects
RNA-binding proteins; CELLULAR control mechanisms; RIBOSOMES; NUCLEOCYTOPLASMIC interactions; NUCLEAR transport; RIBOSOMAL proteins
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-39150-9