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- Title
Optimization of l- DOPA production by Brevundimonas sp. SGJ using response surface methodology.
- Authors
Surwase, Shripad N.; Patil, Sushama A.; Jadhav, Shekhar B.; Jadhav, Jyoti P.
- Abstract
l- DOPA (3,4-dihydroxyphenyl- l-alanine) is an extensively used drug for the treatment of Parkinson's disease. In the present study, optimization of nutritional parameters influencing l- DOPA production was attempted using the response surface methodology ( RSM) from Brevundimonas sp. SGJ. A Plackett- Burman design was used for screening of critical components, while further optimization was carried out using the Box- Behnken design. The optimized levels of factors predicted by the model were pH 5.02, 1.549 g l−1 tryptone, 4.207 g l−1 l-tyrosine and 0.0369 g l−1 CuSO4, which resulted in highest l- DOPA yield of 3.359 g l−1. The optimization of medium using RSM resulted in a 8.355-fold increase in the yield of l- DOPA. The anova showed a significant R2 value (0.9667), model F-value (29.068) and probability (0.001), with insignificant lack of fit. The highest tyrosinase activity observed was 2471 U mg−1 at the 18th hour of the incubation period with dry cell weight of 0.711 g l−1. l- DOPA production was confirmed by HPTLC, HPLC and GC-MS analysis. Thus, Brevundimonas sp. SGJ has the potential to be a new source for the production of l- DOPA.
- Subjects
PARKINSON'S disease; BREVUNDIMONAS; PHENOL oxidase; MATHEMATICAL optimization; RESPONSE surfaces (Statistics)
- Publication
Microbial Biotechnology, 2012, Vol 5, Issue 6, p731
- ISSN
1751-7907
- Publication type
Article
- DOI
10.1111/j.1751-7915.2012.00363.x