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- Title
Identification of a 2-cell stage specific inhibitor of the cleavage of preimplantation mouse embryos synthesized by rat hepatoma cells as 5′-deoxy-5′-methylthioadenosine.
- Authors
Kobayashi, Masayuki; Saito, Koichi; Tamogami, Shigeru; Takashima, Junko; Kasuga, Kano; Kojima, Ikuo
- Abstract
Rat hepatoma Reuber H-35 cells produce a unique compound designated as Fr.B-25, a 2-cell stage-specific inhibitor of the cleavage of preimplantation mouse embryos cultured in vitro. Here, we identified Fr.B-25 as a purine nucleoside, 5′-deoxy-5′-methylthioadenosine (MTA), by mass spectroscopic analysis. All of the biological activities examined of authentic MTA on the development of mouse zygotes were indistinguishable from those of Fr.B-25. The mechanism of MTA action in the development of preimplantation mouse embryos was probably different from those of hypoxanthine and adenosine, which are well-characterized purine nucleosides that act as inhibitors of the cleavage of mouse 2-cell embryos. From the shared molecular and biological properties of Fr.B-25 and MTA, we concluded that Fr.B-25 is MTA. To the best of our knowledge, this is the first delineation of the effect of MTA on the development of preimplantation mammalian embryos cultured in vitro.
- Subjects
METHYLTHIOADENOSINE; EMBRYO transfer; MASS spectrometry; BIOACTIVE compounds; ZYGOTES; MOLECULAR biology; LABORATORY mice
- Publication
Zygote, 2011, Vol 19, Issue 2, p117
- ISSN
0967-1994
- Publication type
Article
- DOI
10.1017/S0967199410000158